Our laboratory utilises human samples collected from pregnant women, cell culture experiments and clinically relevant animal models. We are therefore able to ask the same questions using very different approaches which each complement other aspects of the study.

Specific techniques include: 

  • Biochemical analysis of various cellular processes- enzymatic assays, colourmetic assays, ELISAs and radioimmunoassays
  • Quantification of nutrients in biological samples (preparation for ICPMS)
  • Quantitative real-time PCR for gene expression analysis, assessment of mitochondrial content and genotyping
  • Western blot for protein quantification
  • Co-immunoprecipitation to assess interactions between proteins
  • Immunohistochemistry and immunofluorescence for protein localisation in tissues
  • Histological/pathological staining
  • Metabolic analysis such analysis of glucose tolerance (GTT) in rodents and assessment of renal function
  • Cardiovascular physiology 
  • Placental and kidney stereology

NB: All animal experimentation is subject to approval by the University of Queensland Animal Ethics Committee and adheres to the Australian code for the care and use of animals for scientific purposes (National Health and Medical Research Council, 8th Edition, 2013)