Analytical facilities equipment

Tecan Spark Multimode plate reader

Multimode plate reader
High speed monochromator for absorbance measurements
Nanoquant plate for low volume, calibration free nucleic acid measurements
Luminescence optics for 96, 384 well format glow, flash and multi-colour luminescence applications
Automated cell imaging for cell counting, viability analysis and live cell imaging
Automated incubator with humidity, CO₂ control and a lid lifter
Powerful Magellan software for management of long-term kinetic assays

Multimode plate reader that accepts cuvettes and 6,12,24,48,96 and 384 well microplates
Absorbance, Fluorescence, Time-resolved Fluorometry (TRF) and Luminescence capabilities
Spectral scanning in 1nm increments across the whole spectral range (200-1000)
Measures up to 6 Absorbance wavelengths per read or four wavelength pairs for other read modes
Kinetic, Endpoint, and Well scanning
Temperature control (2 ˚C above ambient to 60˚C). Shaker. Softmax Pro analysis software

Microplate absorbance reader with 12-channel optics for fast measurements (96 well plate in less than 6 seconds)
Ideal for colorimetric ELISAs, kinetic assays and agglutination reading
Tuneable wavelength option
Top and bottom temperature control to minimise evaporation (up to 42˚)
Inbuilt Magellan data analysis software allows an automated analysis, curve fitting and graphical data output to users specifications

Tecan Hydrospeed plate washer Advanced microplate washer suitable for cell assays, bead assays and ELISA.

Highly flexible robotic liquid handling system with easily repositioned carriers for microplates, pipette tips and reagent reservoirs
Intelligent individual liquid detection

Droplet digital PCR system for absolute quantification of target DNA or RNA
Utilises EvaGreen or TaqMan hydrolysis probe based assays
96 well format
Applications include cancer biomarker studies and copy number variation, Genomic SNP detection, Quantification and qualification of next generation sequencing libraries, Gene expression analysis, Environmental monitoring and Food testing for GMOs

Small sample volume (0.5-2 µl) full spectrum UV-Vis spectrophotometer to quantify and assess purity of RNA, DNA and protein
Applications for measurements for UV-Vis, A260, A280, labelled DNA, Protein and labels (Dyes/Chromophores), Protein BCA, Protein Lowry, Protein Bradford, Microarray, Cell cultures
Method Editor for custom methods for utilising formulas and standard curves

Real-time PCR system 96 and 384-well capability
Detection of as low as 1.5-fold changes in singleplex reactions with 10 logs of dynamic range
SYBR and TaqMan chemistries
Multiplexing capabilities
Applications include Gene expression, miRNA profiling, SNP genotyping, Copy number variation, Protein thermal shift, High resolution melt, Pathogen detection

High performance dry chemistry analyser for plasma, serum or whole blood
Simple 3-step procedure
Automated dilution
Biochemical tests for 10 enzymes, 15 general chemistry, 3 electrolytes and immunological test for CRP
Low sample volume (10 µl sample or 50 µl for electrolytes: Na-K-Cl)

High throughput clinical analyser with a large range of 120 assay and applications including clinical chemistry, specific proteins, therapeutic drug monitoring, drug of abuse testing and whole blood HbA1c
Samples can be serum, plasma, urine, hemolysate, whole blood or CSF
Microcups for low volume samples. Utilises ready to use (no mixing) reagent packs

High throughput multiplexing unit for quantification of multiple proteins in a single reaction via magnetic beads
Capable of up to 50 different tests in each reaction volume, with a sensitivity comparable to western blotting and ELISAs
96 well plate format
Utilises both commercially available kits and reagents that are available for user-developed assays

The XFe96 analyser measures glycolytic rates, metabolic and mitochondrial function of live cultured cells (adherent, suspension or 3D cultures), isolated mitochrondria, ex-vivo samples and non-mammalian samples in real time
Highly sensitive, it can analyse as low as 5,000 cells per well in a 96 well format
Automatically measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR)
Capable of 4 independent injections per well with automatic mixing
Applications include dose-response studies and compound screening
The XF prep station complements the analyser by allowing automated washing, incubation and preparation of cells prior to assay with the XFe96 analyser

Laser-based imager
Utilises near-infrared fluorescent dyes allowing high sensitivity and image quality
6-logs of linear dynamic range
Applications include western blotting, in-cell and in –gel westerns, protein and nucleic acid gels, EMSA/Gel shift assays, small animal imaging and tissue sections
Dual-colour capability for simultaneous detection

Flow cytometry analyser
4 lasers; Blue, Red, Violet, and Yellow-green
Simultaneous detection of up to 12-14 fluorescent signals
Fixed alignment flow cell allows high sensitivity, reproducibility and quick start-up
Advanced optics system allows detection of dim fluorescent or rare cell populations
BD FACSDiva software allows automated acquisition of multiple samples and powerful analysis features
FlowJo version 10 is also available for analysis of acquired data