Imaging facilities and analysis equipment

High speed multi-channel fluorescent imaging, with live cell and super resolution TIRF and STORM capabilities. The instrument can also act as a high content imager for screening applications.  

Capable of high speed imaging, deconvolution, DIC, 2-colour TIRF, and super resolution 3D STORM.  Specifications include: 5 Laser lines (405nm, 445nm, 488nm, 561nm, 633nm), two cameras (Andor Zyla 4.2 10-tap and Andor iXon888 EMCCD), adjustable pinhole diameters, and fully incubated for live cell imaging.  Nikon platform with NIS-Elements. 
To download a free viewer - www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer

Objective Lens include: 10x CFI Plan Apo NA 0.45 WD 4.0mm, 20x CFI Plan Apo VC NA 0.75 WD 1.0mm, 40x CFI Plan Apo Lambda NA 0.95 WD 0.25mm, 40x CFI Apo LWD WI (water) LambdaS NA 1.15 WD 0.61mm, 60x CFI Plan Apo WI (water) NA 1.2 WD 0.31mm, 60x Apo TIRF Oil NA 1.49 WD 0.12mm.

Locations: Skerman 2nd Floor (Room 202) or Otto 619

Workstation with Imaris 9.3, Filament tracer, and ImageJ and MatLab plugins. There are two floating licenses available.

Visit www.bitplane.com for more information
Visit www.bitplane.com/learning for video tutorials

Perfect for multi-colour imaging, spectral unmixing and scanning, 3D imaging of sections, 96 well plate imaging, live cell imaging, FRET and FRAP.

Two instruments are available for use.

Specifications include: inverted, incubated confocal microscope - Laser lines include 405nm, 458nm, 476nm, 488nm, 496nm, 514nm, 561nm, 633nm; AOTF; 5 PMT including 2 HyD GaAsP ultra-sensitive detectors; 8K scanner and a 12K resonant scanner.  Objectives include: 10x air NA 0.5, 20x air NA 0.75, 40x air NA 0.85, 63x air NA 0.9, 63x Glycerol NA 1.3, 40x water NA 1.1.

Visit
www.leica-microsystems.com/products/microscope-software/software-for-life-science-research/las-x/#downloads for more information and a free LIF file viewer

High speed multi-channel fluorescent imaging with rapid stage movement enabling whole 96-well plates to be rapidly imaged.  Deconvolution software enables confocal quality images and hightened revolving power.

Specifications include: Two light sources (LED SpectraX and Sola). SpectraX excitation at 390nm, 438nm, 475nm, 513nm, 542nm, 575nm, 633nm; two cameras: Andor sCMOS 5.5 10 tap and Leica DFC450 colour camera.  QUAD filter DAPI/GFP/Cy3/Cy5, LED autofocus, environmental control, motorised XYZ, and structured Illumination for optical sectioning.

Visit www.leica-microsystems.com/products/microscope-software/software-for-life-science-research/las-x/#downloads to download a free viewer

Suitable for fluorescent and brightfield imaging and the recording of instructional videos for teaching and training. Use to create macro-images of whole animals.

Filters include: DAPI, GFP, TxRed (Cy3). Fitted with a monochrome CCD camera and can be fitted with a colour CCD camera when requested. 160x magnification total.  

Visit www.leica-microsystems.com/products/stereo-microscopes-macroscopes/fluorescence/details/product/leica-m205-fa for more information.

100 automated slide scanning capabilities in both 7 colour fluorescence and brightfield modes.  On the fly data analysis such as cell counting and fluroescent intensities. Cloud data storage available.

The Andor Mosaic and the Andor 888 EMCCD camera allows exact ROI to be drawn around cells for FRET, FRAP, Ca²⁺ measurement and photoconversion.  

High resolution scanning upright confocal microscope with 5 laser lines and 4 PMTs, perfect for observation of both fixed and living organisms from above. 

Microscope features include - 405nm, 440nm, 473nm, 559nm, and 633nm laser lines, 3 PMTs + 1 transmitted light PMT.

Objectives include: 10x, 20x, 40x, 60x oil, 100x oil.

Installed Stereoinvestigator and Neurolucida software enables accurate cell counting and neuron tracing of brightfield and fluorescently stained samples. Objectives include - 4x, 10x, 20x, 40x, 60x and 100x. Able to excite the following dyes, DAPI, GFP, Cy3, Alexa Fluor 640.

Dedicated Cloud based Super computer for software function. Software available includes Imaris, NIS elements, ZEN, Neurolucida 360, Stereoinvestigator and Neurolucida, Huygens Deconvolution, Amira Image Analysis and TissueMaker for 3D Image Rendering.   

Suitable for laser microdissection and analysis for DNA, RNA, and protein isolation, whether from archive material or live cells.

Laser capture and Microdissection system - Widefield laser capture.  Objectives include: 5x NA 0.25, 20x NA 0.4, 40x NA 0.6, 63x NA 0.75.