Diagrammatic representation of cycloid arcs test system to estimate lengths of renal tubules
Diagrammatic representation of cycloid arcs test system to estimate lengths of renal tubules (from Walton et al, 2016 ).

Our lab utilises a variety of whole animal/in vivo physiology, in vitro cell culture, molecular biology (protein and mRNA), histology/stereology and behavioural assays. This includes:

  • Quantitative real-time PCR for gene expression analysis
  • Western blot for protein quantification
  • Immunohistochemistry and immunofluorescence for protein localisation in tissues
  • Immunofluorescence of cell types within the pre-implantation blastocyst
  • In situ hybridisation of trophoblast cell types within the immature and definitive placenta
  • Pre-clinical animal models
  • Langendorff heart perfusion assays to measure cardiac function in vitro or isolated perfused heart assay to examine cardiac contractile strength and heart rate in vitro.
  • Pressurised myography to measure physiological function and properties of isolated vessels
  • Radiotelemetry measurement of blood pressure, heart rate and activity in freely moving animals
  • In vitro culture to measure trophoblast outgrowth of pre-implantation blastocysts and their differentiation into invasive giant cells
  • Cell culture of trophoblast stem cells to examine differentiation into downstream lineage restricted subtypes
  • Histology and histopathology
  • Microscopy (including confocal)
  • Stereology for assessment of nephron endowment in the kidney, renal tubule length in the kidney, follicle numbers in the ovary and volume measurements for specific cell compartments in the placenta
  • Non-invasive behavioural testing to assess neurological function in rodents, including measures of anxiety, exploratory behaviour and spatial learning/memory
  • Measurement of hormones in plasma by RIA and ELISA
  • Metabolic assessments such as insulin tolerance test (ITT) and glucose tolerance test (GTT) in rodents
  • DEXA for body composition measurement in rodents

NB: All animal experimentation is subject to approval by the University of Queensland Animal Ethics Committee and adheres to the Australian code for the care and use of animals for scientific purposes (National Health and Medical Research Council, 8th Edition, 2013).