Dr Prajakta Kulkarni
Postdoctoral Research Fellow
School of Biomedical Sciences
Prajakta is part of the Pagan group which studies targeted protein degradation.
Researcher biography
I have a long-term research interest in investigating autophagy pathways and organelle dynamics in a variety of physiological processes and disease models, and to exploit this knowledge for the design of new therapeutics. My research focuses on understanding various signalling pathways involved in autophagy and the work has led to discovering new functions of mTOR signalling, a pathway central to growth and metabolism at the Golgi apparatus.
Journal Articles
Nguyen-Dien, Giang Thanh, Townsend, Brendan, Kulkarni, Prajakta Gosavi, Kozul, Keri-Lyn, Ooi, Soo Siang, Eldershaw, Denaye N, Weeratunga, Saroja, Liu, Meihan, Jones, Mathew JK, Millard, S Sean, Ng, Dominic CH, Pagano, Michele, Bonfim-Melo, Alexis, Schneider, Tobias, Komander, David, Lazarou, Michael, Collins, Brett M and Pagan, Julia K (2024). PPTC7 antagonizes mitophagy by promoting BNIP3 and NIX degradation via SCFFBXL4. EMBO Reports, 25 (8), 3324-3347. doi: 10.1038/s44319-024-00181-y
Kulkarni, Prajakta, Nguyen-Dien, Giang Thanh, Kozul, Keri-Lyn and Pagan, Julia K. (2024). FBXL4: safeguarding against mitochondrial depletion through suppression of mitophagy. Autophagy, 20 (6), 1-3. doi: 10.1080/15548627.2024.2318077
Nguyen‐Dien, Giang Thanh, Kozul, Keri‐Lyn, Cui, Yi, Townsend, Brendan, Kulkarni, Prajakta Gosavi, Ooi, Soo Siang, Marzio, Antonio, Carrodus, Nissa, Zuryn, Steven, Pagano, Michele, Parton, Robert G, Lazarou, Michael, Millard, S Sean, Taylor, Robert W, Collins, Brett M, Jones, Mathew JK and Pagan, Julia K (2023). FBXL4
suppresses mitophagy by restricting the accumulation of
NIX
and
BNIP3
mitophagy receptors. The EMBO Journal, 42 (13) e112767, e112767. doi: 10.15252/embj.2022112767
Zulkefli, Khalisah L., Mahmoud, Ismail S., Williamson, Nicholas A., Gosavi, Prajakta Kulkarni, Houghton, Fiona J. and Gleeson, Paul A. (2021). A role for Rab30 in retrograde trafficking and maintenance of endosome-TGN organization. Experimental Cell Research, 399 (2) 112442, 112442. doi: 10.1016/j.yexcr.2020.112442
Kulkarni‐Gosavi, Prajakta, Makhoul, Christian and Gleeson, Paul A. (2019). Form and function of the Golgi apparatus: scaffolds, cytoskeleton and signalling. FEBS Letters, 593 (17), 2289-2305. doi: 10.1002/1873-3468.13567
Makhoul, Christian, Gosavi, Prajakta and Gleeson, Paul A. (2019). Golgi dynamics: The morphology of the mammalian Golgi apparatus in health and disease. Frontiers in Cell and Developmental Biology, 7 112, 1-7. doi: 10.3389/fcell.2019.00112
Zulkefli, Khalisah L., Houghton, Fiona J., Gosavi, Prajakta and Gleeson, Paul A. (2019). A role for Rab11 in the homeostasis of the endosome-lysosomal pathway. Experimental Cell Research, 380 (1), 55-68. doi: 10.1016/j.yexcr.2019.04.010
Makhoul, Christian, Gosavi, Prajakta, Duffield, Regina, Delbridge, Bronwen, Williamson, Nicholas A. and Gleeson, Paul A. (2019). Intersectin-1 interacts with the golgin GCC88 to couple the actin network and Golgi architecture. Molecular Biology of the Cell, 30 (3), 370-386. doi: 10.1091/mbc.e18-05-0313
Makhoul, Christian, Gosavi, Prajakta and Gleeson, Paul A. (2018). The Golgi architecture and cell sensing. Biochemical Society Transactions, 46 (5), 1063-1072. doi: 10.1042/bst20180323
Gosavi, Prajakta, Houghton, Fiona J., McMillan, Paul J., Hanssen, Eric and Gleeson, Paul A. (2018). The Golgi ribbon in mammalian cells negatively regulates autophagy by modulating mTOR activity. Journal of Cell Science, 131 (3) jcs211987, 1-15. doi: 10.1242/jcs.211987
Gosavi, Prajakta (2018). First person - Prajakta Gosavi. Journal of Cell Science, 131 (3), 1-2. doi: 10.1242/jcs.215640
Gurjar, Mansa, Raychaudhuri, Kumarkrishna, Mahadik, Snehal, Reddy, Divya, Atak, Apurva, Shetty, Trupti, Rao, Kruthi, Karkhanis, Mansi S., Gosavi, Prajakta, Sehgal, Lalit, Gupta, Sanjay and Dalal, Sorab N. (2018). Plakophilin3 increases desmosome assembly, size and stability by increasing expression of desmocollin2. Biochemical and Biophysical Research Communications, 495 (1), 768-774. doi: 10.1016/j.bbrc.2017.11.085
Gosavi, Prajakta and Gleeson, Paul A. (2017). The Function of the Golgi Ribbon Structure – An Enduring Mystery Unfolds!. BioEssays, 39 (11) 1700063, 1-9. doi: 10.1002/bies.201700063
Pols, Maaike S., ten Brink, Corlinda, Gosavi, Prajakta, Oorschot, Viola and Klumperman, Judith (2012). The HOPS proteins hVps41 and hVps39 are required for homotypic and heterotypic late endosome fusion. Traffic, 14 (2), 219-232. doi: 10.1111/tra.12027
Khapare, Nileema, Kundu, Samrat T., Sehgal, Lalit, Sawant, Mugdha, Priya, Rashmi, Gosavi, Prajakta, Gupta, Neha, Alam, Hunain, Karkhanis, Madhura, Naik, Nishigandha, Vaidya, Milind M. and Dalal, Sorab N. (2012). Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis. PLoS ONE, 7 (6) e38561. doi: 10.1371/journal.pone.0038561
Gosavi, Prajakta, Kundu, Samrat T., Khapare, Nileema, Sehgal, Lalit, Karkhanis, Mansi S. and Dalal, Sorab N. (2010). E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly. Cellular and Molecular Life Sciences, 68 (8), 1439-1454. doi: 10.1007/s00018-010-0531-3
Kundu, Samrat T., Gosavi, Prajakta, Khapare, Nileema, Patel, Rachana, Hosing, Amol S., Maru, Girish B., Ingle, Arvind, DeCaprio, James A. and Dalal, Sorab N. (2008). Plakophilin3 downregulation leads to a decrease in cell adhesion and promotes metastasis. International Journal of Cancer, 123 (10), 2303-2314. doi: 10.1002/ijc.23797
Conference Paper
Gurjar, M. C., Raychaudhuri, K., Mahadik, S., Atak, A., Sehgal, L., Karkhanis, M., Gosavi, P. and Dalal, S. N. (2014). Plakophilin3 expression results in an increase in the level of desmosomal components and the formation of calcium independent desmosomes. FEBS EMBO 2014 Conference, Paris, France, 30 August - 4 September 2014. Chichester, West Sussex, United Kingdom: Wiley-Blackwell Publishing.