The Chen laboratory focuses on the link of cell biological properties and functions, including the secretion of hormones, contraction and dilation of cardiomyocytes, or proliferation and apoptosis of cancer cells. We concentrate on hormones, which are important in controlling growth, development, metabolic balance, body mass composition, ageing process, and the plasma glucose levels, such as growth hormone, leptin, insulin, glucagon, and other adipocyte-derived hormones and factors.

The long-term goal is to find an effective way to therapeutically correct growth hormone and insulin deficiency in treating obesity and diabetes. In addition, possible paracrine or autocrine effect of a newly discovered hormone, Ghrelin, in the regulation of uterine endometrial cancer development, is under the investigation in this laboratory. We also investigate the function of cardiomyocytes in chronic heart failure, ischemic and diabetic cardiomyopathy.

The laboratory is using cutting edge techniques including patch clamping, cell image analysis, single cell shortening, and super-sensitive hormone assay method and real-time micro-blood sampling for pulsatile hormone profiles in mouse. Modern molecular biology techniques are also employed in the laboratory for different projects, including GFP-transgenic mouse, shRNA-gene knock down, and real-time PCR.

Lab head

Students

* C Chen is Corresponding Author.

a. Paper in refereed journal          a'. Review in refereed journal

b. Chapter in book

c. Full paper - proceedings of meeting

d. Thesis

2017

  • (a) Jin-Kui Yang, Ying-Ying Wang, Chang Liu, Ting-Ting Shi, Jing Lu, Xi Cao, Fang-Yuan Yang, Jian-Ping Feng, Chen Chen, Li-Nong Ji, Aimin Xu: (2017) Urine Proteome Specific for Eye Damage Can Predict Kidney Damage in Patients With Type 2 Diabetes: A Case-Control and a 5.3-Year Prospective Cohort Study. Diabetes Care, in press.
  • (a)* Xiaolin Chen, Lili Huang, Hwee Y. Tan, Hongzhuo Li, Ying Wan, Michael Cowley, Johannes D. Veldhuis and Chen Chen: (2017) Deficient melanocortin-4 receptor causes abnormal reproductive neuroendocrine profile in female mice. Reproduction, in press.

2016

  • (a) Yibei Zhang, Haiou Qiao, Chen Chen, Zhi-lun Wang, Xiaodong Xia: (2016) Determination of nitrofurans metabolite residues in aquatic products by ultra-performance liquid chromatography-tandem mass spectrometry. Food Chemistry, 192, 612-617
  • (a)* Yan Zhao, Jiezhong Chen, XL Zhang, R Shao, CX Yan, Chen Chen: (2016) Hexarelin protects streptozotocin-induced beta-cell damage through maintaining normal mitochondrial functions. PloS One, 11(2): e0149730. doi:10.1371/journal.pone.0149730.
  • (a’) Frederik J. Steyn, Virginie Tolle, Chen Chen, Jacques Epelbaum: (2016) Neuroendocrine Regulation of Growth Hormone Secretion. Comprehensive Physiology, 6: 687-735.
  • (a) Feifei Cheng, Lu Zhao, Yuanyuan Wu, Tiantian Huang, Gangyi Yang, Zhanyu Zhang, Yijia Wu, Fang Jia, Jinlin Wu, Chen Chen, Dongfang Liu: (2016) Serum vascular endothelial growth factor B is elevated in women with polycystic ovary syndrome and can be decreased with metformin treatment. Clinic Endocrinology (Oxf), 84(3): 386-93.
  • (a)* H.Y. Tan, L. Huang, S.T. Ngo, M. Cowley, J. Greenfield, J.D. Veldhuis, F.J. Steyn, Chen Chen: (2016) Hyperphagia in Male Melanocortin 4 Receptor Deficient Mice Promotes Growth Independent of Growth Hormone. Journal of Physiology, in press 23 August 2016.
  • (a) Wei Li, Yan Liu, Zhi Liu, Zi Wang, Chen Chen, Jing Zhang, Yinshi Sun: (2016) Ginsenoside Rg5 ameliorates cisplatin-induced nephrotoxicity through inhibition of inflammation, oxidative stress, and apoptosis. Nutrients, 8(9): 2016
  • (a) Shel Hwa-Yeo, Victoria Kyle, Paul G. Morris, Sophie Jackman, Lydia Sinnett-Smith, Chen Chen and William H. Colledge: (2016) Visualization of Kiss1 neuron distribution using a Kiss1-CRE transgenic mouse. Journal of Neuroendocrinology, 28: in press.

2015

  • (a) Dmitry A Ovchiennikov, Alejandro Hidalgo, Seung-Kwon Yang, Xinli Zhang, James Hudson, Stuart B Mazzone, Chen Chen, Justin J Cooper-White, Ernst J Wolvetang: (2015) Isolation of contractile cardiomyocytes from human pluripotent stem-cell-derived cardiomyogenic cultures using a human NCX1-EGFP reporter. Stem Cells and Development, 24(1): 11-20.
  • (a) Anne Guillou, Nicola Romano, Frederik Steyn, Karine Abitbol, Paul Le Tissier, Xavier Bonnefont, Chen Chen, Patrice Mollard and Agnès O Martin: (2015) Assessment of lactotroph axis functionality in mice: longitudinal monitoring of PRL secretion by ultrasensitive-ELISA. Endocrinology, 156:1924-1930
  • (a’)* Rasha M. H. Mosa, Zhen Zhang, Renfu Shao, Chao Deng, Jiezhong Chen, Chen Chen: (2015) Implications of ghrelin and hexarelin in diabetes and diabetes-associated heart diseases. Endocrine, 49:307-323.
  • (a’) Jiezhong Chen, Wenyi Gu, Lei Yang, Chen Chen, Renfu Shao, Kewei Xu, Zhi Ping Xu (2015) Nanotechnology in the management of cervical cancer. Reviews in Medical Virology, 25:72-83
  • (a’)* Jiezhong Chen, Renfu Shao, Chen Chen: (2015) Possible therapeutic implication of PDL-1/PD-1 axis in endometrial cancer. Journal of Solid Tumors, 5:10-17
  • (a) KwonJung Yi, KyoungHa So, Yuki Hata, Yutaka Suzuki, Daichi Kato, Kouichi Watanabe, Hisashi Aso, Yoshiyuki Kasahara, Katsuhiko Nishimori, Chen Chen, Kazuo Katoh, Sang-Gun Roh: (2015) The regulation of oxytocin receptor gene expression during adipogenesis. Journal of Neuroendocrinology, 27:335-342.
  • (a)* Catalina Carrasco-Pozo, Martin Gotteland, Rodrigo L. Castillo, Chen Chen: (2015) 3,4-dihydroxyphenylacetic acid, a microbiota-derived metabolite of quercetin, protects against pancreatic β-cells dysfunction induced by high cholesterol. Experimental Cell Research, 334:270-282
  • (a)* T.Y. Xie, S.T. Ngo, J.D. Veldhuis, P.L. Jeffery, L.K. Chopin, M. Tschöp, M.J. Waters, V. Tolle, J. Epelbaum, Chen Chen, F.J. Steyn: (2015) Effect of Deletion of Ghrelin-O-Acyltransferase on the Pulsatile Release of Growth Hormone in Mice. Journal of Neuroendocrinology 27(12):872-86.
  • (a) Lixia Lv, Hewen Chen, Jiaying Sun, Di Lu, Chen Chen, Dongfang Liu: (2015) PRMT1 promotes glucose toxicity-induced β-cell dysfunction by regulating the nucleo-cytoplasmic trafficking of PDX-1 in a FOXO1 dependent manner in INS-1 cells. Endocrine, 49:669-682
  • (a’)* Jiezhong Chen, Tiefeng Xu, Chen Chen: (2015) The critical roles of miR-21 in anti-cancer effects of curcumin. Annals of Translational Medicine, 3:330 - 7

2014

  • (a) Xiaorong Zhou, Zhilun Wang, Jinghong Chen, Wei Wang, Daiqing Song, Siyuan Li, Haojie Yang, Senhai Xue, Chen Chen: (2014) Increased levels of IL-6, IL-1β, and TNF-α in Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Rheumatology International, 34, 995-1004
  • (a’) Jiezhong Chen, Kong-Nan Zhao, Chen Chen: (2014) The role of CYP3A4 in the biotransformation of bile acids and therapeutic implication for cholestasis. Annals of Translational Medicine, 2(1), 7 (1-9)
  • (a’) Jiezhong Chen, Aymen Elfiky, Mei Han, Chen Chen, M. Wasif Saif: (2014) The role of Src in colon cancer and its therapeutic implications. Clinical Colorectal Cancer, 13(1), 5-13
  • (a) Silvia Manzanero, Joanna R. Erion, Tomislav Santro, Frederik J. Steyn, Chen Chen, Thiruma V. Arumugam, and Alexis M. Stranahan: (2014) Intermittent fasting attenuates increases in neurogenesis following ischemia and reperfusion and improves recovery. Journal of Cerebral Blood Flow and Metabolism, 34, 897–905
  • (a) Shengbin Li, Bo Li, Cheng Cheng, Zijun Xiong, Qingbo Liu, Jianghua Lai, Hannah V Carey, Qiong Zhang, Haibo Zheng, Shuguang Wei, Hongbo Zhang, Liao Chang, Shiping Liu, Shanxin Zhang, Bing Yu, Xiaofan Zeng, Yong Huo, Wenhui Nie, Youmin Guo, Teng Chen, Jiuqiang Han, Jian Wang, Jun Wang, Chen Chen, Jiankang Liu, Peter J Stambrook, Ming Xu, Guojie Zhang, Thomas P Gilbert, Huanming Yang, Erich D Jarvis, Jun Yu, Jianqun Yan (2014) Genomic signatures of near-extinction and rebirth of the crested ibis and other endangered bird species. Genome Biology, 15, 557 doi:10.1186/s13059-014-0557-1
  • (a) Rim Hassouna, Philippe Zizzari, Catherine Tomasetto, Johannes D. Veldhuis, Oriane Fiquet, Alexandra Labarthe, Julie Cognet, Frederik Steyn, Chen Chen, Jacques Epelbaum, Virginie Tolle: (2014) An early reduction in GH peak amplitude in preproghrelin deficient mice has a minor impact on linear growth. Endocrinology, 155, 3561-3571
  • (a)* Lili Huang, Hwee Y. Tan, Matthew J. Fogarty, Zane B. Andrews, Johannes D. Veldhuis, Herbert Herzog, Frederik Steyn, Chen Chen: (2014) Actions of NPY, and its Y1 and Y2 receptors on pulsatile growth hormone secretion during the fed and fasted state. Journal of Neuroscience, 34(49), 16309-16319
  • (a)* Chen Chen, Jiezhong Chen, Kong-Nan Zhao: (2014) Signalling Pathways in Anti-cancer Drug Resistance. Current Medicinal Chemistry, 21, 3007-3008 (Editorial)
  • (a)* Jiezhong Chen, Kong-Nan Zhao, Rui Li, Renfu Shao, Chen Chen: (2014) Activation of PI3K/Akt/mTOR Pathway and Dual Inhibitors of PI3K and mTOR in Endometrial Cancer. Current Medicinal Chemistry, 21, 3070-3080

2013

  • (b)* F.J. Steyn, C. Chen: (2013) Adiponectin. In Handbook of Biologically Active Peptides, 2nd Edition, Edited by A.J. Kastin, MD, published by Academic Press/Elsevier, Chapter 130, 983-989.
  • (a’)* Wenyi Gu, Chen Chen, Kong-Nan Zhao: (2013) Obesity-associated endometrial and cervical cancers. Frontiers in Bioscience, E5, 109-118.
  • (a’) Jianghong Wu, Chen Chen. Kong-Nan Zhao: (2013) Phosphatidylinositol 3-kinase signaling as a therapeutic target for cervical cancer. Current Cancer Drug Targets, 13(2), 143-156.
  • (a)* Yufeng Zhao, Li Wang, Jianhua Qiu, Dingjun Zha, Qiang Sun, Chen Chen: (2013) Linoleic acid stimulates [Ca2+]i increase in rat pancreatic beta-cells through both membrane receptor- and intracellular metabolite-mediated pathways. PloS One, 8, e60255.
  • (a’) Jiezhong Chen, Mengna Chi, Chen Chen, Xu-Dong Zhang: (2013) Obesity and melanoma: Exploring molecular links. Journal of Cellular Biochemistry, 114, 1955-1961.
  • (a)* F.J. Steyn, Y. Wan, J. Clarkson, J.D. Veldhuis, A. E. Herbison & Chen Chen: (2013) Development of methodology for, and assessment of pulsatile luteinizing hormone secretion in juvenile and adult male mice. Endocrinology, 154, 4939-4945
  • (a) Zhao Xu, Zhou Wang, Jian-jun Li, Chen Chen, Ping-chuan Zhang, Lu Dong, Jing-hong Chen, Qun Chen, Xiao-tian Zhang, Zhi-lun Wang: (2013) Protective effects of selenium on oxidative damage and oxidative stress related gene expression in rat liver under chronic poisoning of arsenic. Food and Chemical Toxicology, 58, 1-7
  • (a’)* Jiezhong Chen, Renfu Shao, Xu-Dong Zhang and Chen Chen: (2013) Applications of nanotechnology in the diagnosis and treatment of melanoma. International Journal of Nanomedicine, 8, 2677-2688
  • (a’)* Jiezhong Chen, Ross Crawford, Chen Chen and Yin Xiao: (2013) The key regulatory roles of the PI3K/Akt signalling pathway in the functionalities of mesenchymal stem cells and applications in tissue regeneration. Tissue Engineering, 19(6), 526-528
  • (a)* Yufeng Zhao, Li Wang, Dingjun Zha, Li Qiao, Lianjun Lu, Jun Yu, Ping Qu, Qiang Xun, Jianha Qiu, Chen Chen: (2013) GW9508 inhibits insulin secretion by activating KATP channels in rat pancreatic beta-cells. Journal of Molecular Endocrinology, 51, 69–77
  • (a)* F.J. Steyn, T.Y. Xie, L. Huang, S.T. Ngo, J.D. Veldhuis, M. Waters, C. Chen (2013) Increased adiposity and insulin correlates with the suppression of pulsatile GH secretion during weight gain. Journal of Endocrinology, 218, 233-244
  • (a)* Jenny N. T. Fung, Penny Jeffery, John Lee, Inge Seim, Andreas Obermair, Lisa  K. Chopin and Chen Chen: (2013) Silencing of Ghrelin Receptor Expression Inhibits Endometrial Cancer Cell Growth in vitro and in vivo. American Journal of Physiology, 305, E305-E313
  • (a)* H.Y. Tan, L. Huang, D. Simmons, J.D. Veldhuis, F.J. Steyn and Chen Chen: (2013) Hypothalamic distribution of Somatostatin (Sst) mRNA expressing neurons relative to pubertal and adult changes in pulsatile GH secretion in mice. Journal of Neuroendocrinology, 25, 910-919
  • (a)* Yufeng Zhao, Dingjun Zha, Li Wang, Li Qiao, Lianjun Lu, Lin Mei, Chen Chen, Jianha Qiu: (2013) Phenotypic characterization of GPR120-expressing cells in the interstitial tissue of pancreas. Tissue and Cell, 45, 421-427
  • (a)* F.J. Steyn, K. Lee, M.J. Fogarty, J.D. Veldhuis, P.A. McCombe, M.C. Bellingham, S.T. Ngo, and Chen Chen: (2013) Growth hormone secretion is correlated with neuromuscular innervation rather than motor neuron number in early-symptomatic male Amyotrophic Lateral Sclerosis mice. Endocrinology, 154, 4695-4706
  • (a) H. Zou, X.L. Gan, L.J. Linghu, Chen Chen, L.N. Hu, Y. Zhang: (2013) Intense nanosecond pulsed electric fields promote cancer cell apoptosis through centrosome-dependent pathway involving reduced level of PLK1. European Review for Medical and Pharmacological Sciences, 17, 152-160
  • (a) Lai-Ling Du, Jia-Zhao Xie, Xiang-Shu Cheng, Xiao-Hong Li, Fan-Li Kong, Xia Jiang, Zhi-Wei Ma, Jian-Zhi Wang, Chen Chen, Xin-Wen Zhou: (2013) Activation of sirtuin1 attenuates cerebral ventricular administration of streptozotocin-induced tau hyperphosphorylation in rat Hippocampi and cognitive injuries. AGE, 36, 613–623

2012

  • (a)* F.J. Steyn, J.W. Leong, L. HUANG, H.Y. Tan, T.Y. Xie, C. Nelson, M.J. Waters, J.D. Veldhuis, J. Epelbaum, Chen Chen: (2012) Growth hormone does not modulate the early fasting induced release of free fatty acids in mice. Endocrinology, 153:273-282.
  • (a’)* Sean-Kwon Yang, Frederik Steyn, Chen Chen: (2012) Influence of Membrane Ion Channel in Pituitary Somatotrophs by Hypothalamic Regulators. Cell Calcium, 51:231-239.
  • (a)* F.J. Steyn, S.T. Ngo, J.D. Lee, J.W. Leong, J.D. Veldhuis, P.A. McCombe, Chen Chen, M.C. Bellingham: (2012) Impairments to the GH-IGF1 axis in hSOD1G93A mice give insight into possible mechanisms of GH dysregulation in patients with Amyotrophic Lateral Sclerosis. Endocrinology, 153:3735-3746.
  • (b)* Jenny N.T. Fung, Chen Chen: (2012) Endometrial Cancer: Diagnosis, treatment and prevention. In Horisons in Cancer Research. Editor: Hiroto S. Watanabe. Vol 49 (Chapter I), 1-32.
  • (a’)* Xinli Zhang, Chen Chen: (2012) A new insight of mechanisms, diagnosis and treatment of diabetic cardiomyopathy. Endocrine, 41:398-409.
  • (a)* Ya Tuo, Dandan Feng, Dengfeng Wang, Jian Sun, Shenbin Li, Chen Chen: (2012) Long-term in vitro treatment of INS-1 rat pancreatic β-cells by unsaturated free fatty acids protects cells from gluco- and lipo-toxicities through activation of GPR40 receptors. Clin Exp Physiol Pharmacol, 39:423-428.
  • (a) Jyothsna R Rao, Damien J Keating, Chen Chen, Helena C Parkington: (2012) Adiponectin increases insulin content and cell proliferation in MIN6 cells via PPARγ-dependent and PPARγ -independent mechanisms. Diabetes, Obesity and Metabolism, 14:983-989.
  • (a)* Yi Ma, Lin Zhang, Joshua N. Edwards, Bradley S. Launikonis, Chen Chen: (2012) Growth Hormone Secretagogues protect mouse cardiomyocytes from in vitro ischemia/reperfusion injury through regulation of intracellular calcium. PLoS One, 7(4): e35265.
  • (a) Chen JH, Xue S, Li S, Wang ZL, Yang H, Wang W, Song D, Zhou X, Chen Chen: (2012) Oxidant damage in Kashin-Beck disease and a rat Kashin-Beck disease model by employing T-2 toxin treatment under selenium deficient conditions. J Orthop Res. 30:1229-1237.
  • (a)* Yi Ma, Lin Zhang, Bradley S. Launikonis, Chen Chen: (2012) Growth hormone secretagogues preserve action potential of mouse cardiomyocytes isolated from in vitro ischemic/reperfusion heart. Endocrinology, 153: 5480-5490.
  • (a)* Xiangbin Xu, Fan Ding, Jinjiang Pang, Xue Gao, Rong-Kun Xu, Wei Hao, Ji-Min Cao, Chen Chen: (2012) Chronic administration of hexarelin attenuates cardiac fibrosis in the spontaneously hypertensive rat. Am J Physiol (HC), 303, H703-H711.
  • (a)* L. Huang, F.J. Steyn, H.Y. Tan, T.Y. Xie, J.D. Veldhuis, C. Chen: (2012) The decline in pulsatile GH secretion through early adulthood in mice is exacerbated by dietary-induced weight gain. Endocrinology, 153, 4380-4388.
  • (a) Liang-han Ling, Ouda Khammy, Melissa Byrne, Fatemah Amirahmadi, Anna Foster, Gefeng Li, Linda Zhang, Cris dos Remedios, Chen Chen, David M. Kaye: (2012) Irregular Rhythm Adversely Influences Calcium Handling in Ventricular Myocardium: Implications for the Interaction Between Heart Failure and Atrial Fibrillation. Circulation Heart Failure, 5, 786-793.

2011

  • (b)* Yufeng Zhao, Chen Chen: (2011) Adipsin as an adipokine. In Adipokines, Edit Victor R. Preedy. by Science Publishers, New Hampshire, USA; Chapter 3, page 20-28.
  • (a)* Ya Tuo, Dengfeng Wang, Shenbin Li, Chen Chen: (2011) Long-term exporure of INS-1 rat insulinoma cells to linoleic acid and glucose in vitro affects cell viability and function through mitochondrial-mediated pathways. Endocrine, 39:128-138.
  • (a)* F. J. Steyn, L. Huang, S.T. Ngo, J.W. Leong, H.Y. Tan, T.Y. Xie, A.F. Parlow, J.D. Veldhuis, M.J. Waters, and Chen Chen: (2011) Development of a method for the determination of pulsatile growth hormone secretion in mice. Endocrinology, 152:3165-3171.
  • (a)* Xuefeng Han, Yunlong Zhu, Yufeng Zhao, Chen Chen: (2011) Ghrelin reduces voltage-gated calcium currents in GH3 cells via cyclic GMP pathways. Endocrine, 40:228-236.
  • (a) Heng Zou, Dengfeng Wang, Xiaoling Gan, Long Jiang, Chen Chen, Lina Hu, Yi Zhang: (2011) Low TWEAK expression is correlated to the progression of squamous cervical carcinoma. Gynecologic Oncology, 123:123-128.
  • (a) Dandan Feng, Seung-Kwon Yang, Loudes Catherine, Simon Axelle, Culler Michael, Alvear-Perez Rodrigo, Llorens-Cortes Catherine, Chen Chen, Epelbaum Jacques, Gardette Robert: (2011) Ghrelin and Obestatin modulate Growth Hormone-Releasing Hormone release and synaptic inputs onto growth hormone-releasing hormone neurons. European Journal of Neuroscience, 34:732-744.
  • (a)* Li Wang, Yufeng Zhao, Baosong Gui, Rongguo Fu, Feng Ma, Jun Yu, Ping Qu, Lei Dong, Chen Chen: (2011) Acute stimulation of glucagon secretion by linoleic acid results from GPR40 activation and [Ca2+]i increase in pancreatic islet alpha-cells. Journal of Endocrinology, 210:173-179.

Honours

  1. Metabolic regulation by GH pulsatile profiles in mouse under positive or negative energy balance
  2. Cell function analysis of insulin cells with insulin resistance
  3. Regulation of Pim-1 expression in diabetic cardiomyopathy in rat

PhD

  1. Development of iPSC techniques in the treatment of motor neuron disease
  2. Islet cell dysfunction in type 2 diabetes
  3. Metabolic regulation of GH profiles through peripheral and neuroendocrine system

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