Dr Leon Hugo

Honorary Senior Fellow

School of Biomedical Sciences

l.hugo@uq.edu.au

Researcher biography

Dr Leon Hugo is a medical entomologist conducting research into the biology of mosquitoes, host-parasite interactions and the development of novel control strategies against arthropod-borne viruses (arboviruses). Dr Hugo is an Honorary Associate Professor through the School of Biomedical Sciences and is also the Academic Lead of the Mosquito Control Laboratory at QIMR Berghofer medical research institute, Brisbane. Dr Hugo has developed and applied experimental mosquito models of arbovirus infection for over 15 years to establish the risk of arbovirus transmission in Australia and new biological controls. Dr Hugo works with physical containment/biosecurity level (PC/BSL) 2 and 3 arboviruses, including dengue, Zika, Japanese encephalitis and chikungunya viruses and performs fundamental research on Wolbachia bacteria and Insect-specific virus (ISV) microbial control agents. Dr Hugo has published 60+ research articles with over 160 collaborators, from academic, medical, government and corporate sectors, across 18 countries. Topic areas his publications have had greatest impact were Japanese Encephalitis and Socioeconomic Impact, Wolbachia's Role in Mosquito Population Replacement and Insecticide Resistance Mechanisms in Aedes aegypti.

Dr Hugo's team maintains a living library of native and exotic mosquito species and conducts infection and transmission studies in state-of-the-art PC2 and PC3 insectaries. Research themes including mosquito vector competence, climate change impacts on mosquito-borne disease transmission risk, mosquito and arbovirus surveillance and genomics and novel control strategies, including biological control and the use of endectocides.

Publications

Journal Articles (61)

Conference Papers (8)

Journal Articles

Al-Amin, Hasan Mohammad, Gyawali, Narayan, Graham, Melissa, Alam, Mohammad Shafiul, Lenhart, Audrey, Xi, Zhiyong, Rašić, Gordana, Beebe, Nigel W., Hugo, Leon E. and Devine, Gregor J. (2025). Fitness compatibility and dengue virus Inhibition in a Bangladeshi strain of Aedes aegypti infected with the Wolbachia strain wAlbB. Scientific Reports, 15 (1) 13425, 1-18. doi: 10.1038/s41598-025-98093-x

Hussain, Mazhar, Etebari, Kayvan, Parry, Rhys H., Khromykh, Alexander A., Hugo, Leon E. and Asgari, Sassan (2025). Transcriptomic response of mosquitoes to Japanese encephalitis virus and identification of its potential entry factors. npj Viruses, 3 (1) 68, 68-1. doi: 10.1038/s44298-025-00151-8

Fritzer, Andrea, Suhrbier, Andreas, Hugo, Leon E., Tang, Bing, Devine, Greg, Jost, Sandra and Meinke, Andreas L. (2025). Assessment of the transmission of live-attenuated chikungunya virus vaccine VLA1553 by Aedes albopictus mosquitoes. Parasites & Vectors, 18 (1) 171. doi: 10.1186/s13071-025-06789-w

Peterson, Alyssa J., Hall, Roy A., Harrison, Jessica J., Hobson-Peters, Jody and Hugo, Leon E. (2024). Unleashing nature’s allies: comparing the vertical transmission dynamics of insect-specific and vertebrate-infecting flaviviruses in mosquitoes. Viruses, 16 (9) 1499, 1499. doi: 10.3390/v16091499

Pruszynski, Catherine A., Buckner, Eva A., Burkett-Cadena, Nathan D., Hugo, Leon E., Leal, Andrea L. and Caragata, Eric P. (2024). Estimation of population age structure, daily survival rates, and potential to support dengue virus transmission for Florida Keys Aedes aegypti via transcriptional profiling. PLoS Neglected Tropical Diseases, 18 (8) e0012350. doi: 10.1371/journal.pntd.0012350

Rawle, Daniel J., Hugo, Leon E., Cox, Abigail L., Devine, Gregor J. and Suhrbier, Andreas (2024). Generating prophylactic immunity against arboviruses in vertebrates and invertebrates. Nature Reviews Immunology, 24 (9), 621-636. doi: 10.1038/s41577-024-01016-6

Hugo, Leon E., van Huyssteen, Karla, Oloniniyi, Olamide, Donnelly, Laura, Conn, Anna, Collins, Katharine A., Mitchell, Hayley, McCarthy, James S. and Macdonald, Joanne (2024). Rapid low-resource detection of Plasmodium falciparum in infected Anopheles mosquitoes. Frontiers in Tropical Diseases, 5 1287025. doi: 10.3389/fitd.2024.1287025

Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”. Journal of Virology, 97 (5) e0045123, 1-3. doi: 10.1128/jvi.00451-23

Al‐Amin, Hasan Mohammad, Gyawali, Narayan, Graham, Melissa, Alam, Mohammad Shafiul, Lenhart, Audrey, Hugo, Leon E., Rašić, Gordana, Beebe, Nigel W. and Devine, Gregor J. (2023). Insecticide resistance compromises the control of Aedes aegypti in Bangladesh. Pest Management Science, 79 (8), 2846-2861. doi: 10.1002/ps.7462

Furuya-Kanamori, Luis, Gyawali, Narayan, Mills, Deborah J., Mills, Christine, Hugo, Leon E, Devine, Gregor J. and Lau, Colleen L. (2023). Immunogenicity of a single fractional intradermal dose of Japanese encephalitis live attenuated chimeric vaccine. Journal of Travel Medicine, 30 (2) taac122, 1-8. doi: 10.1093/jtm/taac122

Ahmed, Madeeha, Pollak, Nina M., Hugo, Leon E., van den Hurk, Andrew F., Hobson-Peters, Jody and Macdonald, Joanne (2022). Rapid molecular assays for the detection of the four dengue viruses in infected mosquitoes. Gates Open Research, 6 81, 1-20. doi: 10.12688/gatesopenres.13534.2

Farokhinejad, Fahimeh, Li, Junrong, Hugo, Leon E., Howard, Christopher B., Wuethrich, Alain and Trau, Matt (2022). Detection of Dengue Virus 2 with single infected mosquito resolution using yeast affinity bionanofragments and plasmonic SERS nanoboxes. Analytical Chemistry, 94 (41), 14177-14184. doi: 10.1021/acs.analchem.2c02012

Yakob, Laith, Hu, Wenbiao, Frentiu, Francesca D, Gyawali, Narayan, Hugo, Leon E, Johnson, Brian, Lau, Colleen, Furuya-Kanamori, Luis, Magalhaes, Ricardo Soares and Devine, Gregor (2022). Japanese encephalitis emergence in Australia: the potential population at risk. Clinical Infectious Diseases, 76 (2), 335-337. doi: 10.1093/cid/ciac794

Ahmed, Madeeha, Nath, Nisa Suraj, Hugo, Leon E., Devine, Gregor J., Macdonald, Joanne and Pollak, Nina M. (2022). Rapid detection of kdr mutation F1534C in Aedes aegypti using recombinase polymerase amplification and lateral flow dipsticks. Pesticide Biochemistry and Physiology, 187 105209, 1-7. doi: 10.1016/j.pestbp.2022.105209

Hugo, Leon E., Rašić, Gordana, Maynard, Andrew J., Ambrose, Luke, Liddington, Catherine, Thomas, Callum J. E., Nath, Nisa Suraj, Graham, Melissa, Winterford, Clay, Wimalasiri-Yapa, B M C Randika, Xi, Zhiyong, Beebe, Nigel W. and Devine, Gregor J. (2022). Wolbachia wAlbB inhibit dengue and Zika infection in the mosquito Aedes aegypti with an Australian background. PLoS Neglected Tropical Diseases, 16 (10) e0010786, e0010786. doi: 10.1371/journal.pntd.0010786

Bishop, Cameron, Hussain, Mazhar, Hugo, Leon E. and Asgari, Sassan (2022). Analysis of Aedes aegypti microRNAs in response to Wolbachia wAlbB infection and their potential role in mosquito longevity. Scientific Reports, 12 (1) 15245, 1-17. doi: 10.1038/s41598-022-19574-x

Torres, Francisco J., Parry, Rhys, Hugo, Leon E., Slonchak, Andrii, Newton, Natalee D., Vet, Laura J., Modhiran, Naphak, Pullinger, Brody, Wang, Xiaohui, Potter, James, Winterford, Clay, Hobson-Peters, Jody, Hall, Roy A. and Khromykh, Alexander A. (2022). Reporter flaviviruses as tools to demonstrate homologous and heterologous superinfection exclusion. Viruses, 14 (7) 1501, 1501. doi: 10.3390/v14071501

Islam, Nazmul, Xu, Chang, Lau, Colleen L., Mills, Deborah J., Clark, Justin, Devine, Gregor J., Hugo, Leon E., Gyawali, Narayan, Thalib, Lukman and Furuya-Kanamori, Luis (2022). Persistence of antibodies, boostability, and interchangeability of Japanese encephalitis vaccines: A systematic review and dose-response meta-analysis. Vaccine, 40 (26), 3546-3555. doi: 10.1016/j.vaccine.2022.04.079

Furuya-Kanamori, Luis, Gyawali, Narayan, Mills, Deborah J., Hugo, Leon E., Devine, Gregor J. and Lau, Colleen L. (2022). The emergence of Japanese encephalitis in Australia and the implications for a vaccination strategy. Tropical Medicine and Infectious Disease, 7 (6) 85, 85. doi: 10.3390/tropicalmed7060085

Peng, Nias Y. G., Amarilla, Alberto A., Hugo, Leon E., Modhiran, Naphak, Sng, Julian D. J., Slonchak, Andrii, Watterson, Daniel, Setoh, Yin Xiang and Khromykh, Alexander A. (2022). The distinguishing NS5-M114V mutation in American Zika virus isolates has negligible impacts on virus replication and transmission potential. PLoS Neglected Tropical Diseases, 16 (5) e0010426, 1-17. doi: 10.1371/journal.pntd.0010426

Beebe, Nigel W., Pagendam, Dan, Trewin, Brendan J., Boomer, Andrew, Bradford, Matt, Ford, Andrew, Liddington, Catherine, Bondarenco, Artiom, de Barro, Paul J., Gilchrist, Joshua, Paton, Christopher, Staunton, Kyran M., Johnson, Brian, Maynard, Andrew J., Devine, Gregor J., Hugo, Leon E., Rasic, Gordana, Cook, Helen, Massaro, Peter, Snoad, Nigel, Crawford, Jacob E., White, Bradley J., Xi, Zhiyong and Ritchie, Scott A. (2021). Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia. Proceedings of the National Academy of Sciences of the United States of America, 118 (41) e2106828118, e2106828118. doi: 10.1073/pnas.2106828118

Newton, Natalee D., Hardy, Joshua M., Modhiran, Naphak, Hugo, Leon E., Amarilla, Alberto A., Bibby, Summa, Venugopal, Hariprasad, Harrison, Jessica J., Traves, Renee J., Hall, Roy A., Hobson-Peters, Jody, Coulibaly, Fasséli and Watterson, Daniel (2021). The structure of an infectious immature flavivirus redefines viral architecture and maturation. Science Advances, 7 (20) eabe4507, 1-12. doi: 10.1126/sciadv.abe4507

Rigby, Lisa M., Johnson, Brian J., Rašić, Gordana, Peatey, Christopher L., Hugo, Leon E., Beebe, Nigel W., Hartel, Gunter F. and Devine, Gregor J. (2021). The presence of knockdown resistance mutations reduces male mating competitiveness in the major arbovirus vector, Aedes aegypti. PLoS Neglected Tropical Diseases, 15 (2) e0009121, 1-13. doi: 10.1371/journal.pntd.0009121

Ekwudu, O’mezie, Marquart, Louise, Webb, Lachlan, Lowry, Kym S., Devine, Gregor J., Hugo, Leon E. and Frentiu, Francesca D. (2020). Effect of serotype and strain diversity on dengue virus replication in Australian mosquito vectors. Pathogens, 9 (8) 668, 668-14. doi: 10.3390/pathogens9080668

Gyawali, Narayan, Murphy, Amanda K., Hugo, Leon E. and Devine, Gregor J. (2020). A micro-PRNT for the detection of Ross River virus antibodies in mosquito blood meals: A useful tool for inferring transmission pathways. PLoS One, 15 (7) e0229314, 1-13. doi: 10.1371/journal.pone.0229314

Rigby, Lisa M., Rašić, Gordana, Peatey, Christopher L., Hugo, Leon E., Beebe, Nigel W. and Devine, Gregor J. (2020). Identifying the fitness costs of a pyrethroid-resistant genotype in the major arboviral vector Aedes aegypti. Parasites & Vectors, 13 (1) 358, 358. doi: 10.1186/s13071-020-04238-4

Pickering, Paul, Hugo, Leon E., Devine, Gregor J., Aaskov, John G. and Liu, Wenjun (2020). Australian Aedes aegypti mosquitoes are susceptible to infection with a highly divergent and sylvatic strain of dengue virus type 2 but are unlikely to transmit it. Parasites and Vectors, 13 (1) 240, 1-7. doi: 10.1186/s13071-020-04091-5

Slonchak, Andrii, Hugo, Leon E., Freney, Morgan E., Hall-Mendelin, Sonja, Amarilla, Alberto A., Torres, Francisco J., Setoh, Yin Xiang, Peng, Nias Y. G., Sng, Julian D. J., Hall, Roy A., van den Hurk, Andrew F., Devine, Gregor J. and Khromykh, Alexander A. (2020). Zika virus noncoding RNA suppresses apoptosis and is required for virus transmission by mosquitoes. Nature Communications, 11 (1) 2205, 2205. doi: 10.1038/s41467-020-16086-y

Johnson, Brian J., Hugo, Leon E., Churcher, Thomas S., Ong, Oselyne T. W. and Devine, Gregor J. (2020). Mosquito age grading and vector-control programmes. Trends in Parasitology, 36 (1), 39-51. doi: 10.1016/j.pt.2019.10.011

Gyawali, Narayan, Taylor-Robinson, Andrew W., Bradbury, Richard S., Huggins, David W., Hugo, Leon E., Lowry, Kym and Aaskov, John G. (2019). Identification of the source of blood meals in mosquitoes collected from north-eastern Australia. Parasites and Vectors, 12 (1) 198, 198. doi: 10.1186/s13071-019-3455-2

Hugo, Leon E., Stassen, Liesel, La, Jessica, Gosden, Edward, Ekwudu, O'mezie, Winterford, Clay, Viennet, Elvina, Faddy, Helen M., Devine, Gregor J. and Frentiu, Francesca D. (2019). Vector competence of Australian Aedes aegypti and Aedes albopictus for an epidemic strain of Zika virus. PLoS Neglected Tropical Diseases, 13 (4) e0007281, e0007281. doi: 10.1371/journal.pntd.0007281

Pasay, Cielo J., Yakob, Laith, Meredith, Hannah R., Stewart, Romal, Mills, Paul C., Dekkers, Milou H., Ong, Oselyne, Llewellyn, Stacey, Hugo, R. Leon E., McCarthy, James S. and Devine, Gregor J. (2019). Treatment of pigs with endectocides as a complementary tool for combating malaria transmission by Anopheles farauti (s.s.) in Papua New Guinea. Parasites and Vectors, 12 (1) 124. doi: 10.1186/s13071-019-3392-0

Hugo, R. Leon E. and Birrell, Geoff W. (2018). Proteomics of Anopheles Vectors of Malaria. Trends in Parasitology, 34 (11), 961-981. doi: 10.1016/j.pt.2018.08.009

Sikulu-Lord, Maggy T., Devine, Gregor J., Hugo, Leon E. and Dowell, Floyd E. (2018). First report on the application of near-infrared spectroscopy to predict the age of Aedes albopictus Skuse. Scientific Reports, 8 (1) 9590, 9590. doi: 10.1038/s41598-018-27998-7

Osei-Amo, S, Hussein, M, Asad, S, Hugo, L and Asgari, S (2018). Wolbachia-induced transcription factor GATA4 suppresses ovary-specific genes blastoderm-specific protein 25D and imaginal disc growth factor. Insect Molecular Biology, 27 (3), 295-304. doi: 10.1111/imb.12371

Asad, Sultan, Hussain, Mazhar, Hugo, Leon, Osei-Amo, Solomon, Zhang, Guangmei, Watterson, Daniel and Asgari, Sassan (2018). Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti. PLoS Neglected Tropical Diseases, 12 (4) e0006405, e0006405. doi: 10.1371/journal.pntd.0006405

Setoh, Yin Xiang, Prow, Natalie A., Peng, Nias, Hugo, Leon E., Devine, Gregor, Hazlewood, Jessamine E., Suhrbier, Andreas and Khromykh, Alexander A. (2017). De novo generation and characterization of new Zika virus isolate using sequence data from a microcephaly case. mSphere, 2 (3) e00190-17, 1-11. doi: 10.1128/mSphereDirect.00190-17

Griffin, Paul, Pasay, Cielo, Elliott, Suzanne, Sekuloski, Silvana, Sikulu, Maggy, Hugo, Leon, Khoury, David, Cromer, Deborah, Davenport, Miles, Sattabongkot, Jetsumon, Ivinson, Karen, Ockenhouse, Christian and McCarthy, James (2016). Safety and reproducibility of a clinical trial system using induced blood stage Plasmodium vivax infection and its potential as a model to evaluate malaria transmission. PLoS Neglected Tropical Diseases, 10 (12) e0005139, e0005139. doi: 10.1371/journal.pntd.0005139

Hugo, Leon E., Prow, Natalie A., Tang, Bing, Devine, Greg and Suhrbier, Andreas (2016). Chikungunya virus transmission between Aedes albopictus and laboratory mice. Parasites and Vectors, 9 (1) 555. doi: 10.1186/s13071-016-1838-1

Sikulu-Lord, Maggy T., Milali, Masabho P., Henry, Michael, Wirtz, Robert A., Hugo, Leon E., Dowell, Floyd E. and Devine, Gregor J. (2016). Near-infrared spectroscopy, a rapid method for predicting the age of male and female wild-type and Wolbachia infected Aedes aegypti. PLoS Neglected Tropical Diseases, 10 (10) e0005040, e0005040.1-e0005040.11. doi: 10.1371/journal.pntd.0005040

Ulrich, Jill N., Beier, John C., Devine, Gregor J. and Hugo, Leon E. (2016). Heat sensitivity of wMel Wolbachia during Aedes aegypti development. PLoS Neglected Tropical Diseases, 10 (7) e0004873, e0004873. doi: 10.1371/journal.pntd.0004873

Kho, Elise A., Hugo, Leon E., Lu, Guangjin, Smith, David D. and Kay, Brian H. (2016). Effects of Larval Nutrition on Wolbachia-Based Dengue Virus Interference in Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology, 53 (4), 894-901. doi: 10.1093/jme/tjw029

Sikulu-Lord, Maggy T., Maia, Marta F., Milali, Masabho P., Henry, Michael, Mkandawile, Gustav, Kho, Elise A., Wirtz, Robert A., Hugo, Leon E., Dowell, Floyd E. and Devine, Gregor J. (2016). Rapid and non-destructive detection and identification of two strains of Wolbachia in Aedes aegypti by near-infrared spectroscopy. PLoS Neglected Tropical Diseases, 10 (6) e0004759, e0004759. doi: 10.1371/journal.pntd.0004759

Sikulu, Maggy T., Monkman, James, Dave, Keyur A., Hastie, Marcus L., Dale, Patricia E., Kitching, Roger L., Killeen, Gerry F., Kay, Brian H., Gorman, Jeffry J. and Hugo, Leon E. (2015). Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi. Data in Brief, 4, 461-467. doi: 10.1016/j.dib.2015.07.007

Sikulu, Maggy T., Monkman, James, Dave, Keyur A., Hastie, Marcus L., Dale, Patricia E., Kitching, Roger L., Killeen, Gerry F., Kay, Brian H., Gorman, Jeffery J. and Hugo, Leon E. (2015). Proteomic changes occurring in the malaria mosquitoes Anopheles gambiae and Anopheles stephensi during aging. Journal of Proteomics, 126, 234-244. doi: 10.1016/j.jprot.2015.06.008

Sikulu, Maggy T., Majambere, Silas, Khatib, Bakar O., Ali, Abdullah S., Hugo, Leon E. and Dowell, Floyd E. (2014). Using a near-infrared spectrometer to estimate the age of Anopheles mosquitoes exposed to pyrethroids. PLoS One, 9 (3) e90657, e90657.1-e90657.6. doi: 10.1371/journal.pone.0090657

Hugo, Leon E., Jeffery, Jason A. L., Trewin, Brendan J., Wockner, Leesa F., Nguyen Thi Yen, Nguyen Hoang Le, Le Trung Nghia, Hine, Emma, Ryan, Peter A. and Kay, Brian H. (2014). Adult Survivorship of the Dengue Mosquito Aedes aegypti Varies Seasonally in Central Vietnam. PLoS Neglected Tropical Diseases, 8 (2) e2669, e2669.1-e2669.12. doi: 10.1371/journal.pntd.0002669

Hugo, Leon E., Monkman, James, Dave, Keyur A., Wockner, Leesa F., Birrell, Geoff W., Norris, Emma L., Kienzle, Vivian J., Sikulu, Maggy T., Ryan, Peter A., Gorman, Jeffery J. and Kay, Brian H. (2013). Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission. PLoS One, 8 (3) e58656, e58656.1-e58656.10. doi: 10.1371/journal.pone.0058656

Sikulu, Maggy, Dowell, Kayla M., Hugo, Leon E., Wirtz, Robert A., Michel, Kristin, Peiris, Kamaranga H. S., Moore, Sarah, Killeen, Gerry F. and Dowell, Floyd E. (2011). Evaluating RNAlater ® as a preservative for using near-infrared spectroscopy to predict Anopheles gambiae age and species. Malaria Journal, 10 (1) 186. doi: 10.1186/1475-2875-10-186

Hugo, LE, Kay, BH, O'Neill, SL and Ryan, PA (2010). Investigation of environmental influences on a transcriptional assay for the prediction of age of Aedes aegypti (Diptera: Culicidae) mosquitoes. Journal of Medical Entomology, 47 (6), 1044-1052. doi: 10.1603/ME10030

Hugo, Leon E., Cook, Peter E., Johnson, Petrina H., Rapley, Luke P., Kay, Brian H., Ryan, Peter A., Ritchie, Scott A. and O'Neill, Scott L. (2010). Field validation of a transcriptional assay for the prediction of age of uncaged Aedes aegypti mosquitoes in northern Australia. PLoS Neglected Tropical Diseases, 4 (2) e608, e608-1-e608-9. doi: 10.1371/journal.pntd.0000608

Sikulu, Maggy, Killeen, Gerry F., Hugo, Leon E., Ryan, Peter A., Dowell, Kayla M., Wirtz, Robert A., Moore, Sarah J. and Dowell, Floyd E. (2010). Near-infrared spectroscopy as a complementary age grading and species identification tool for African malaria vectors. Parasites and Vectors, 3 (1) 49. doi: 10.1186/1756-3305-3-49

Moreira, LA, Iturbe-Ormaetxe, I, Jeffery, JA, Lu, GJ, Pyke, AT, Hedges, LM, Rocha, BC, Hall-Mendelin, S, Day, A, Riegler, M, Hugo, LE, Johnson, KN, Kay, BH, McGraw, EA, van den Hurk, AF, Ryan, PA and O'Neill, SL (2009). A Wolbachia Symbiont in Aedes aegypti Limits Infection with Dengue, Chikungunya, and Plasmodium. Cell, 139 (7), 1268-1278. doi: 10.1016/j.cell.2009.11.042

Hugo, R. L., Quick-Mile, S., Kay, B.H. and Ryan, P.A. (2008). Evaluations of Mosquito Age Grading Techniques Based on Morphological Changes. Journal of Medical Entomology, 45 (3), 353-369. doi: 10.1603/0022-2585(2008)45[353:EOMAGT]2.0.CO;2

Cook, P. E., Hugo, L. E., Iturbe-Ormaetxe, I., Williams, C. R., Chenoweth, S. F., Ritchie, S. A., Ryan, P. A., Kay, B. H., Blows, M. W. and O'Neill, S. L. (2007). Predicting the age of mosquitoes using transcriptional profiles. Nature Protocols, 2 (11), 2796-2806. doi: 10.1038/nprot.2007.396

Cook, P. E., Hugo, L. E., Iturbe-Ormaetxe, I., Williams, C. R., Chenoweth, S. F., Ritchie, S. A., Ryan, P. A., Kay, B. H., Blows, M. W. and O'Neill, S. L. (2006). The use of transcriptional profiles to predict adult mosquito age under field conditions. Proceedings of the National Academy of Sciences of the United States of America, 103 (48), 18060-18065. doi: 10.1073/pnas.0604875103

Hugo, Leon E., Kay, Brian H., Eaglesham, Geoff K., Holling, Neil and Ryan, Peter A. (2006). Investigation of cuticular hydrocarbons for determining the age and survivorship of Australasian mosquitoes. American Journal of Tropical Medicine and Hygiene, 74 (3), 462-474. doi: 10.4269/ajtmh.2006.74.462

Hugo, L. E., Kay, B. H. and Ryan, P. A. (2003). Autogeny in Ochlerotatus vigilax (Diptera : Culicidae) from southeast Queensland, Australia. Journal of Medical Entomology, 40 (6), 897-902. doi: 10.1603/0022-2585-40.6.897

Conference Papers

Slonchak, Andrii, Hugo, Leon E., Freney, Morgan, Amarilla, Alberto A., Hall-Mendelin, Sonja, Yan, Kexin, Torres, Francisco, Setoh, Yin Xiang, Peng, Nias, Chaggar, Harman K., Hurk, Andrew Van Den, Wolvetang, Ernst, Suhrbier, Andreas, Devine, Gregor J. and Khromykh, Alexander A. (2020). Zika Virus sfRNA plays an essential role in the infection of insects and mammals. Viruses 2020—Novel Concepts in Virology, Barcelona, Spain, 5–7 February 2020. Basel, Switzerland: MDPI. doi: 10.3390/proceedings2020050112

Hugo, Leon E., Graham, Melissa C., Hussain, Malik, Lowry, Kym, Devine, Gregor J. and Aaskov, John G. (2020). Dengue virus defective interfering particles in mosquitoes. 68th Annual Meeting of the American Society for Tropical Medicine and Hygiene (ASTMH), National Harbor, MD United States, 20-24 November 2019. Deerfield, IL United States: American Society of Tropical Medicine and Hygiene. doi: 10.4269/ajtmh.abstract2019

Wu, Chen, Fernandes, Jill, Devine, Greg and Hugo, Leon (2018). Characterization of Wolbachia infections from native Australian mosquito vectors. 67th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene (ASTHM), New Orleans, Los Angeles, 28 October - 1 November 2018. Deerfield, IL, United States: American Society of Tropical Medicine and Hygiene.

Kuzma, Rebecca, Grant, Jessica, Pilotte, Nils, Hugo, Leon, Devine, Gregor, Lau, Colleen and Williams, Steven (2018). A novel quantitative PCR assay for the detection of Ross River virus. 67th Annual Meeting of the American Society of Tropical Medicine and Hygiene (ASTHM), New Orleans, LA, United States, 28 October - 1 November 2018. Arlington, VA, United States: American Society of Tropical Medicine and Hygiene.

Hugo, Leon, Stassen, Liesel, Devine, Gregor and Frentiu, Francesca (2018). Relative vector competence of Aedes aegypti and Ae. albopictus for an epidemic strain of Zika virus under simulated field conditions. 67th Annual Meeting of the American Society of Tropical Medicine and Hygiene (ASTHM), New Orleans, LA, United States, 28 October - 1 November 2018. Arlington, VA, United States: American Society of Tropical Medicine and Hygiene.

Hugo, Leon E., Darbro, Jonathan, Kho, Elise, Gyawali, Narayan and Devine, Greg (2017). Detection of mammalian antibodies against Ross River virus in mosquito blood meals and potential for arbovirus surveillance. 65th Annual Meeting of the American Society of Tropical Medicine and Hygiene (ASTMH), Atlanta, GA, United States, 13 - 16 November 2016. Arlington, VA, United States: American Society of Tropical Medicine and Hygiene.

Pasay, Cielo, Mills, Paul, Dekkers, Milou, Stewart, Romal, Hugo, Leon, Ong, Oselyne, Wu, Chen, Devine, Greg and McCarthy, James (2017). Investigating the activity of the macrocyclic lactones ivermectin and moxidectin against malaria vectors. 66th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene (ASTMH), Baltimore, MA United States, 5-9 November 2017. McLean, VA, United States: American Society of Tropical Medicine and Hygiene.

Sikulu-Lord, Maggy, Wirtz, Robert, Hugo, Leon, Ulrich, Jill, Devine, Gregor, Masabho, Milali P., de Freitas, Rafael and Dowell, Floyd (2017). Next generation mosquito surveillance technique: the near infra-red spectroscopy. 66th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene (ASTMH), Baltimore, Maryland USA, 05-09 November 2017. Deerfield, IL United States: American Society of Tropical Medicine and Hygiene.